In-vitro toxicology

In vitro toxicology has progressed hugely over the past decades and is now seen as a science in itself. In line with the 3Rs concept, ERBC performs ICH and OECD compliant tests to identify toxicology events in nonwhole animal models (cell cultures, 3D models…).

Rapid, reproducible and cost-effective, these assays contribute to enhance R&D productivity and minimize the use of animals.

Skin corrosion

Membrane barrier test method for skin corrosion (Corrositex®)
Human skin model test (Episkin™)

Skin irritation

Human skin model test (Episkin™)

Acute oral systemic toxicity

Balb/C 3T3 neutral red uptake

Phototoxicity

Balb/C 3T3 cell phototoxicity assay (neutral red uptake)

Cytotoxicity

Test by direct or indirect contact in clone 929, Balb/C 3T3, V79

In vitro wound healing

Cell proliferation and migration in primary normal human keratinocytes

Hepatotoxicity

Cytotoxicity in primary rat hepatocytes
HepG2 cell screening test

Nephrotoxicity

LLC-RK1 cell screening test

Hæmatotoxicity

Colony forming unit granulocyte/macrophage assay
Red blood cell haemolysis test

In vitro cancer screen

Endpoints

Mitochondrial Reductase Activity (MTT Staining)
Sulforhodamine B Uptake

Cell line panels

Non-Small Cell Lung Cancer (NSCLC): A549, NCI-H23, NCI-H460
Colon cancer: HCT-116, HT29, SW-620
Ovarian cancer: OVCAR-3, SK-OV-3
Prostate cancer: PC-3, DU-145
Breast tumor: MCF7, MDA-MB-231, HS 578T